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KMID : 0617319970060010083
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Journal of Pharmacetical Sceiences Ewha Womans University
1997 Volume.6 No. 1 p.83 ~ p.88
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Molecular Genetic Analysis of nm23-H1 Gene in Korean Colorectal Cancers
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Park, Minkoo
Yoon, Wan Hee/Lee, Kong-Joo/Kim, Eunhee
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Abstract
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We have performed molecular genetic analysis of the nm23-H1 gene on 17 Korean colorectal cancer patients. The cDNA, intron Ⅰ, and intron Ⅳ of nm23-H1 gene were used as probes to examine the loss of heterozygosity (LOH) in Korean colorectal tumor. Southern blot analysis using three probes suggested that functional and/or non-functional nm23-H1 homologous gene copies might exist in the human genome. Among the three probes, intron Ⅳ probe seemed to be the most specific one under our hybridization condition. Southern blot analysis with colon cancer cell lines showed 7.6 kb and/or 2.3 kb bands. None of the colon cancer cell lines displayed loss of both bands. Southern blot analysis of Korean colorectal tumors and corresponding normal mucosas with BglⅡ using intron Ⅳ probe demonstrated 3 size bands: 7.6, 4.3, and 2.3 kb. This deserves attention because only 7.6 and 2.3 kb bands appeared in Caucasian and Japanese cases. The identity of the 4.3 kb band is not yet understood. Three out of 17 Korean colon cancer patients (17.6%) showed LOH when 7.6 kb and 2.3 kb bands were considered. This frequency is slightly higher than that in Japanese colorecrtal tumors, where 10.3% of LOH was reported (Okada et al., 1994). However this frequency is lower than those of two independent studies, in which the LOH of colorectal tumors were 52.3% and 22%, respectively (Cohn et al., 1991; Leone et al., 1991a).
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